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ECIS is used in over 160 labs worldwide and ECIS data has been published in a growing list of over 275 peer-reviewed publications.
ECIS electrically monitors cell morphological changes and provides quantitative, real-time measurements of cell motility, attachment and spreading, and barrier function. Experimental protocols include studies of cell-integrin interactions, metastatic invasion, signal transduction, and cell migration (via automated wound/healing).
The ECIS Application Webinar series will review the topics listed below in 20 minute, web-based, interactive seminars. To obtain a copy of the presentation slides, please send an email request to info@biophysics.com.
All Webinars are Presented by:
Dr. Charles Keese,
President
Cell Invasion / Extravasation Assays – 11:00 AM EST September 7, 2010
This webinar will review ECIS as it is used to monitor endothelial monolayers that have been challenged with metastatic cell lines. Extravasation of these layers can be followed in real time to provide quantitative data regarding the metastatic potential of the cancer cells. To obtain a copy of the presentation slides please email info@biophysics.com.
Automated Cell Migration – 11:00 AM EST September 21, 2010
The ECIS Wound Healing Assay replaces the traditional "scratch" or "scrape" assay. Instead of disrupting the cell layer mechanically with a toothpick, needle or pipette tip and following the migration of cells to "heal" the wound with a microscope, we employ electric signals to both wound and monitor the healing process. This webinar will review the basic principles of ECIS and then present quantitative data regarding this new assay. To obtain a copy of the presentation slides please email info@biophysics.com.
Barrier Function Assays – 11:00 AM EST October 5, 2010
This webinar will review ECIS and then show how complex impedance measurements can be used to evaluate the barrier function (permeability) of confluent endothelial monolayers. The ability of ECIS data to be mathematically modeled will be briefly introduced. To obtain a copy of the presentation slides please email info@biophysics.com.
Real-time Electroporation and Monitoring – 11:00 AM EST October 19, 2010
ECIS can be used to electroporate cells and then immediately follow the results of the electroporation in real-time. This unique feature of the ECIS technology will be described in this webinar and sample data presented. To obtain a copy of the presentation slides please email info@biophysics.com.
Cell Attachment and Spreading Measurements – 11:00 AM EST November 2, 2010
The webinar will review ECIS and then present quantitative data gathered in real time regarding the attachment and spreading behavior of cells in vitro.
To view a brief video click here: Cell Attachment and Spreading Measurments (20MB file).
Signal Transduction Assays – 11:00 AM EST November 16, 2010
Data will be presented showing the ability of ECIS to monitor signal transduction in label-free whole-cell assays. The morphological changes responsible for the impedance changes will be further evaluated by modeling the complex impedance data. To obtain a copy of the presentation slides please email info@biophysics.com.
Toxicology with ECIS – 11:00 AM EST December 7, 2010
This webinar will review ECIS and then present data showing how ECIS impedance measurements respond to varying concentrations of toxic compounds. To obtain a copy of the presentation slides please email info@biophysics.com.
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