Traditional methods for measuring transepithelial electrical resistance (TEER) have been limited to single timepoint measurements and require removal of the cells from incubated conditions. With the ECIS 8-well Transfilter Array (8wTFA) that connects to the 16-well array station, TEER is collected continously in real-time while the cells remain incubated, making this an essential tool for accurate TEER quantification.
Measure the dynamics of barrier formation to maturity in real-time.
The 8wTFA conducts TEER measurements while the cells remain inside of the incubator.
No need to worry about variability of measurements from moving electrodes, these are fixed!
The ECIS® 8-well Transfilter Array (8wTFA) allows researchers to measure transepithelial electrical resistance (TEER) on standard cell culture 24-well inserts using Electric Cell-substrate Impedance Sensing (ECIS®). The 8wTFA connects with the ECIS® Z-Theta 16-well array station and can monitor TEER continuously in real-time without having to remove the cells from the incubator.
ECIS® Z-theta measures complex impedance across multiple AC frequencies and separates impedance into series resistance (R) and capacitance (C). For TEER, low frequency series resistance and capacitive reactance are isolated and then converted to an RC parallel circuit providing the real resistance for TEER calculations. High frequency capacitance can also be used with the 8wTFA to determine the point of confluence.
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